Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Tauroursodeoxycholic acid inhibits experimental colitis by preventing early intestinal epithelial cell death.

doi: 10.1038/labinvest.2014.117

Figure Lengend Snippet: Figure 1 Disease activity parameters in TUDCA- and placebo-treated mice during DSS-induced colitis. (a) Mortality (censored data), (b) weight loss, (c) colon shortening, and (d–f) the expression of colonic proinflammatory cytokine IL1B protein levels, and Cxcl2 and Tnf mRNA expression during the course of DSS-induced colitis. NZ6 at each time point. *Po0.05, **Po0.01.

Article Snippet: DSS Induction and TUDCA Treatment Fifty-eight 8- to 10-week-old male C57BL/6 mice were purchased from Harlan (Harlan Laboratories, Horst, The Netherlands) and housed in a temperature-controlled room at 201C with a 12:12-h light-dark cycle.

Techniques: Activity Assay, Expressing

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Tauroursodeoxycholic acid inhibits experimental colitis by preventing early intestinal epithelial cell death.

doi: 10.1038/labinvest.2014.117

Figure Lengend Snippet: Figure 2 Histology and intestinal epithelial barrier function in TUDCA- and placebo-treated mice. (a) Histological scoring and representative images ( 200) of the colon of TUDCA- and placebo-treated mice at day 10 post DSS. (b) The number of bacteria found in the spleen at days 0 (no DSS), 7 and 14 post DSS and (c) quantitative analysis of periodic acid-Schiff staining in the colon of TUDCA- and placebo-treated animals during the course of DSS-induced colitis. (d) Representative images ( 1000) of periodic acid-Schiff-stained colon sections of TUDCA- and placebo-treated animals at days 0 (no DSS), 3, 10, and 14 post DSS. NZ6 at each time point. *Po0.05. NS, not significant; CFU, colony forming units.

Article Snippet: DSS Induction and TUDCA Treatment Fifty-eight 8- to 10-week-old male C57BL/6 mice were purchased from Harlan (Harlan Laboratories, Horst, The Netherlands) and housed in a temperature-controlled room at 201C with a 12:12-h light-dark cycle.

Techniques: Bacteria, Staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Tauroursodeoxycholic acid inhibits experimental colitis by preventing early intestinal epithelial cell death.

doi: 10.1038/labinvest.2014.117

Figure Lengend Snippet: Figure 3 Apoptotic markers in freshly isolated colonic epithelial cells following DSS administration in TUDCA- and placebo-treated mice. (a) Caspase-3 activity assessed using a DEVD-fluorogenic assay and (b) Bcl2 mRNA levels measured by qRT-PCR. NZ6 at each time point. *Po0.05, **Po0.01.

Article Snippet: DSS Induction and TUDCA Treatment Fifty-eight 8- to 10-week-old male C57BL/6 mice were purchased from Harlan (Harlan Laboratories, Horst, The Netherlands) and housed in a temperature-controlled room at 201C with a 12:12-h light-dark cycle.

Techniques: Isolation, Activity Assay, Quantitative RT-PCR

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Tauroursodeoxycholic acid inhibits experimental colitis by preventing early intestinal epithelial cell death.

doi: 10.1038/labinvest.2014.117

Figure Lengend Snippet: Figure 6 The effect of TUDCA on TNF-induced caspase-3 activation and apoptosis. (a) HT29 cells were stimulated with a combination of TNF and IFNG (TNF) for 24 h and treated with 1, 5, or 10 mM TUDCA, or an equal volume of medium. In the preincubation setting, TUDCA was added for 6 h, TUDCA was washed off and then the cells were stimulated with TNF for another 24 h. N ¼ 6 for each condition and the experiment was repeated four times. (b) Electron microscopic images ( 7000) of a monolayer of T84 cells stimulated with TNF with and without 10 mM TUDCA. Apoptotic bodies are indicated with arrows. **Po0.01 and ***Po0.001. RLU, relative light units.

Article Snippet: DSS Induction and TUDCA Treatment Fifty-eight 8- to 10-week-old male C57BL/6 mice were purchased from Harlan (Harlan Laboratories, Horst, The Netherlands) and housed in a temperature-controlled room at 201C with a 12:12-h light-dark cycle.

Techniques: Activation Assay